.png){kind=logo}
Brief description
This record contains data collected on the RV Southern Surveyor, in the Indian ocean during September 2012. Samples were collected between Fremantle and Darwin. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and retrieved chlorophyll a estimate, and the absorption coefficient for particulate (a/p) and detrital or non-algal (a/d) components of the water column. A total of 9 stations were sampled.Lineage
Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. Pigment analysis 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes.
The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and
refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of pigments were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.
The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and
refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop. Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001). The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of pigments were determined from standards (Sigma, USA or DHI, Denmark). Spectral absorption 4 litres of sample water was filtered through a 25 mm glass fibre filter (Whatman GF/F) and the filter then stored flat in liquid nitrogen until analysis. Optical density spectra for total particulate matter were obtained using a GBC 916 UV/VIS dual beam spectrophotometer equipped with an integrating sphere.
Notes
CreditThis voyage was a collaboration between CSIRO and the University of Western Australia
Credit
Eric Raes (CSIRO O&A
Eric Raes (CSIRO O&A
Credit
Floreat)
Floreat)
Credit
Lesley Clementson (CSIRO O&A
Lesley Clementson (CSIRO O&A
Credit
Hobart)
Hobart)
Data time period: 2012-09-07 to 2012-09-13
text: westlimit=114.265; southlimit=-30.93; eastlimit=122.291; northlimit=-14.792
text: uplimit=-6; downlimit=-6
Subjects
CTD |
CTDs (Conductivity-Temperature-Depth Profilers) |
Coastal Waters (Australia) | West Australia Coast, WA |
Earth Science | Oceans | Ocean Chemistry | Chlorophyll |
Earth Science | Oceans | Ocean Chemistry | Pigments |
Earth Science | Oceans | Ocean Optics | Ocean Color |
Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll |
Fluorometers |
Global / Oceans | East Indian Ocean |
Global / Oceans | Indian Ocean |
HPLC (High Performance Liquid Chromatography) |
Marine Features (Australia) | Australian EEZ |
Marine National Facility |
Marine debris distribution in the Eastern Indian Ocean and Timor Sea. |
Niskin Bottles |
Practical salinity of the water body |
Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it |
Pressure (measured variable) in the water body exerted by overlying sea water only |
Research Transit: SS2012_T06 |
Ship: Southern Surveyor |
Spectrophotometers |
Temperature of the water body |
WfO Biogeochemistry |
oceans |
research vessel |
User Contributed Tags
Login to tag this record with meaningful keywords to make it easier to discover
Other Information
Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Data Link)
uri :
https://aesop.csiro.au/
Data also available via IMOS and AODN portal (Data Link)
uri :
https://portal.aodn.org.au/
Marine National Facility (Documentation Link)
uri :
https://mnf.csiro.au/
This work is licensed under a Creative Commons Attribution 4.0 International License (Documentation Link)
Identifiers
- Local : Anzlic Identifier: ANZCW0306014640
- Local : Marlin Record Number: 14640
- global : 26948eeb-7db7-3d25-e053-08114f8c3c5b
