Brief description
This record describes HPLC pigment data measured from 21 sampling stations in the King George River region located in the Kimberley region of Western Australia. The samples were collected on the Research Vessel Solander, Trip 5755 (SOL_5755) during June 6-11,2013. The voyage began on June 3, 2014 in Broome, Western Australia, and ended on June 14, 2013 in Darwin, Northern Territory. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments.Lineage
Progress Code: completedThe samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours.
After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes.
The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop.
Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001).
The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark).
Notes
CreditJohn Keesing (O&A, IOMRC)
Joanna Strzelecki (O&A, IOMRC)
James McLaughlin (O&A, IOMRC)
Dongyan Liu (Chinese Academy of Sciences)
Data time period: 06 06 2013 to 11 06 2013
text: westlimit=127.237; southlimit=-13.79; eastlimit=127.358; northlimit=-14.041; projection=WGS 84 (EPSG:4326)
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