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Brief description
This record describes HPLC pigment data measured from 21 sampling stations in the King George River region located in the Kimberley region of Western Australia. The samples were collected on the Research Vessel Solander, Trip 5755 (SOL_5755) during June 6-11,2013. The voyage began on June 3, 2014 in Broome, Western Australia, and ended on June 14, 2013 in Darwin, Northern Territory. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments.Lineage
Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the vessel and stored under cool and dark conditions until filtering took place on land. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory. For pigment analysis, 4 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube.
The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours.
After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes.
The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop.
Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001).
The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark).
The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours.
After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes.
The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters Alliance high performance liquid chromatography system, comprising a 2695XE separations module with column heater and refrigerated autosampler and a 2996 photo-diode array detector. Immediately prior to injection the sample extract was mixed with a buffer solution (90:10 28 mM tetrabutyl ammonium acetate, pH 6.5 : methanol) within the sample loop.
Pigments were separated using a Zorbax Eclipse XDB-C8 stainless steel 150 mm x 4.6 mm ID column with 3.5 µm particle size (Agilent Technologies) with gradient elution as described in Van Heukelem and Thomas (2001).
The separated pigments were detected at 436 nm and identified against standard spectra using Waters Empower software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma, USA or DHI, Denmark).
Notes
CreditJohn Keesing (O&A, IOMRC)
Credit
Joanna Strzelecki (O&A, IOMRC)
Joanna Strzelecki (O&A, IOMRC)
Credit
James McLaughlin (O&A, IOMRC)
James McLaughlin (O&A, IOMRC)
Credit
Dongyan Liu (Chinese Academy of Sciences)
Dongyan Liu (Chinese Academy of Sciences)
Data time period: 06 06 2013 to 11 06 2013
text: westlimit=127.237; southlimit=-13.79; eastlimit=127.358; northlimit=-14.041; projection=WGS 84 (EPSG:4326)
Subjects
CTD |
CTDs (Conductivity-Temperature-Depth Profilers) |
Coastal Waters (Australia) | West Australia Coast North, WA |
Earth Science | Oceans | Ocean Chemistry | Chlorophyll |
Earth Science | Oceans | Ocean Chemistry | Pigments |
Earth Science | Oceans | Ocean Optics | Ocean Color |
Earth Science | Terrestrial Hydrosphere | Water Quality/Water Chemistry | Chlorophyll |
Filtration Systems |
Fluorometers |
HPLC (High Performance Liquid Chromatography) |
Marine Features (Australia) | Kimberley Coast, WA |
Niskin Bottles |
Practical salinity of the water body |
Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it |
Pressure (measured variable) in the water body exerted by overlying sea water only |
Regional Seas | Timor Sea |
Spectrophotometers |
States, Territories (Australia) | Western Australia |
Temperature of the water body |
WfO Biogeochemistry |
environment |
oceans |
research vessel |
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Other Information
Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Australian-waters Earth Observation Phytoplankton-type Products (AEsOP))
uri :
https://aesop.csiro.au/
Data also available via IMOS and AODN portal (IMOS and AODN portal)
Identifiers
- global : 31ad7cf3-c6d7-48ca-9dbb-68ddabd99e2b
