Brief description
This record contains data collected from 42 CTD stations between 11 September and 01 October 2000 aboard the RV Marion Dufresne , between Indonesia and Perth. The data can be used for Ocean Colour sensor validation. Parameters measured are the concentration of chlorophyll and carotenoid pigments and retrieved chlorophyll a estimate.Lineage
Progress Code: completed
Maintenance and Update Frequency: notPlanned
Statement: Water samples were taken on-board the RV Marion Dufresne. Samples were filtered onboard. Samples were analysed and QC procedures were carried out in the Ocean Colour Laboratory.
Pigment analysis:
6 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures.
Pigment analysis:
6 litres of sample water was filtered through a 47 mm glass fibre filter (Whatman GF/F) and then stored in liquid nitrogen until analysis. To extract the pigments, the filters were cut into small pieces and covered with 100% acetone (3 mls) in a 10 ml centrifuge tube. The samples were vortexed for about 30 seconds and then sonicated for 15 minutes in the dark. The samples were then kept in the dark at 4 °C for approximately 15 hours. After this time 200 µL water was added to the acetone such that the extract mixture was 90:10 acetone:water (vol:vol) and sonicated once more for 15 minutes. The extracts were centrifuged to remove the filter paper and then filtered through a 0.2 µm membrane filter (Whatman, anatope) prior to analysis by HPLC using a Waters high performance liquid chromatograph, comprising a 600 controller, 717 plus refrigerated autosampler and a 996 photo-diode array detector. Pigments were separated using a stainless steel 25 cm x 4.6 mm I.D. column packed with ODS2 of 5 µm particle size (SGE) with gradient elution as described in Wright et al., [1991]. The separated pigments were detected at 436 nm and identified against standard spectra using Waters Millenium software. Concentrations of chlorophyll a, chlorophyll b, b,b-carotene and b,e-carotene in sample chromatograms were determined from standards (Sigma) and all other pigment concentrations were determined from standards of purified pigments isolated from algal cultures.
Notes
CreditFunding and support for this work were received from NASA - John Marra
Credit
Lamont-Doherty Earth Observatory of Columbia University, USA
Lamont-Doherty Earth Observatory of Columbia University, USA
Credit
French organisations LODYC and IFRTP
French organisations LODYC and IFRTP
Credit
Lesley Clementson
Lesley Clementson
Credit
Thomas Moore
Thomas Moore
Data time period: 2000-09-11 to 2000-10-01
text: westlimit=110; southlimit=-32; eastlimit=118; northlimit=-8
Subjects
Air Sea Isotopic & Biochemical Modelling 1997-2000 |
Chlorophyll |
CTD |
CTDs (Conductivity-Temperature-Depth Profilers) |
Coastal Waters (Global) | Indonesia Coast |
EARTH SCIENCE |
Fluorometers |
Global / Oceans | Indian Ocean |
HPLC (High Performance Liquid Chromatography) |
Niskin Bottles |
OCEAN CHEMISTRY |
Ocean Color |
OCEAN OPTICS |
OCEANS |
Pigments |
Practical salinity of the water body |
Pressure (measured variable) in the water body exerted by overlying sea water and any medium above it |
Pressure (measured variable) in the water body exerted by overlying sea water only |
Ship: Marion Dufresne (RV) |
Spectrophotometers |
Temperature of the water body |
WfO Biogeochemistry |
oceans |
research vessel |
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Other Information
Access all data - Australian-waters Earth Observation Phytoplankton-type Products (AEsOP) (Data Link)
uri :
https://aesop.csiro.au/
Data also available via IMOS and AODN portal (Data Link)
uri :
https://portal.aodn.org.au/
This work is licensed under a Creative Commons Attribution 4.0 International License (Documentation Link)
Identifiers
- global : 3cf18c5b-bce6-4221-bce4-5406c28ac879
- Local : Marlin Record Number: 6443
- Local : Anzlic Identifier: ANZCW0306006443
