Data from: A standardised protocol for measuring bioelectrical impedance in green turtles (Chelonia mydas)

Full description

Dataset of the manuscript "A standardised protocol for measuring bioelectrical impedance in green turtles (Chelonia mydas)" (Kophamel et al., 2022).

Bioelectrical impedance analysis (BIA) is gaining popularity in wildlife studies as a portable technology for immediate and non-destructive predictions of body composition components, such as fat-free and fat masses. Successful application of BIA for field-based research requires the identification and control of potential sources of error, as well as the creation and adherence to a standardised protocol for measurement. The aim of our study was to determine sources of error and to provide a standardisation protocol to improve measurement precision of BIA on juvenile green turtles (Chelonia mydas) (n=35). We assessed the effects of altered environmental temperature (20-30ºC), postprandial state (2-72 hours), and time out of the water (two hours) on five impedance parameters (Rinf, R0, R50, PhA50, and Ri) using a bioimpedance spectroscopy device. Technical reproducibility of measurements and inter-animal variability were also assessed.

Dataset includes parameters measured and an explanation of the codes used.

Formats available: .csv, .xlsx, .ods

Software/equipment used to create/collect the data: A handheld impedance spectroscopy analyser (SFB7, Impedimed, Brisbane, QLD, Australia) measured resistance and reactance at 256 logarithmically-spaced frequencies in the range 3-1000 kHz. Impedance measurements were recorded in replicate (n=10) with each measurement taking 800 ms.

Software/equipment used to manipulate/analyse the data: Recorded impedance values were analysed using Bioimp software (v5.4.0.3, ImpediMed Ltd, Brisbane, QLD, Australia), that calculates resistance (R, ohm), reactance (Xc, ohm) and phase angle (PhA, arctangent Xc/R, degrees) at each frequency and uses Cole analysis to obtain estimated resistance at infinite frequency (Rinf) and at zero frequency (R0) (Cornish et al., 1993) (for further details please refer to the manufacturer's website: https://www.impedimed.com/).

The full materials and methods are available in the Open Access publication from the Related publications link below.