Brief description
In August 2004, an experiment was conducted to test the effects of temperature on larval development and metamorphosis for the solitary coral, Fungia repanda, collected from a patch reef slope in northern Okinawa, Japan. Three hours after fertilisation, the embryos from a single male and female colony cross were transferred into triplicate 2 litre plastic beakers, maintained in incubators set at 26, 28 and 30°C (±0.5°C).Larvae were sampled from each beaker on days 3 and 4 post-spawning and transferred to six-well cell culture plates (8-12 larvae per well, n = 3 wells) containing 10 ml of 1 µm filtered sea water (FSW) and a small (<5 mm) chip of unidentified crustose coralline algae (CCA) to initiate metamorphosis. Culture wells containing coral larvae and CCA were maintained at the same temperatures as the larval cultures (±0.3°C). The percentage of larvae undergoing early metamorphosis was assessed at 12 h.In November 2007, a second experiment tested the effects of temperature on larval development and metamorphosis of Acropora millepora, Acropora spathulata and Symphyllia recta from Viper Reef on the Great Barrier Reef. Acropora millepora was sampled from the reef slope, while the other two species were collected from the upper reef flat. Three hours after fertilisation, 400 embryos from at least three colonies were transferred into three separate 1 litre polycarbonate bottles containing 700 ml FSW. The bottles containing the embryos were floated in triplicate computer-controlled flow-through aquaria at 26, 28, 30 and 32°C (±0.3°C) and 80 µmol quanta/m²/s (12:12 h light:dark cycle).Each day larvae were transferred from the floating containers into six-well plates (10-15 per well) containing 10 ml FSW. In total, 9 wells were used for each temperature treatment each day (3 wells of larvae from each water bath x 3 water baths for each temperature). A 5 mm x 5 mm x 3 mm chip of the CCA Neogoniolithon fosliei was added to each well to induce metamorphosis. The larvae were then transferred into incubators set to 26, 28, 30 and 32°C (±0.3°C). Early metamorphosis was assessed after 18 h.Larvae of Acropora millepora, Acropora spathulata and Symphyllia recta were also mass cultured at 28°C in 500 litre indoor flow-through aquaria 80 µmol quanta/m²/s (12:12 h light:dark cycle), to investigate the possible effects of temperature on metamorphosis (not development). Each day larvae were transferred from the cultures into six-well cell culture plates (10-15 larvae per well, n = 3 wells) containing 10 ml FSW. The larvae were then transferred into incubators set to 26, 28, 30 and 32°C (±0.3°C) and early metamorphosis assessed following the addition of small CCA chips. Laboratory experiments were conducted to determine the effects of temperature on the rate of attaining competence to settle and metamorphosis in azooxanthellate larvae of reef-building coral species.Lineage
Maintenance and Update Frequency: notPlannedNotes
CreditHeyward, Andrew J, Dr (Principal Investigator)
Modified: 13 03 2024
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Plasticity of larval pre-competency in response to temperature: observations on multiple broadcast spawning coral species: Heyward AJ and Negri AP (2010) Plasticity of larval pre-competency in response to temperature: observations on multiple broadcast spawning coral species. Coral Reefs 29: 631-636.
local : articleId=8383
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