Data

Images showing a rapid differential staining technique for Fusarium pseudograminearum in cereal tissues during crown rot infections

University of Southern Queensland
Noel Knight (Principal investigator) Prof Mark W. Sutherland (Associated with)
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ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=http://eprints.usq.edu.au/29371/&rft.title=Images showing a rapid differential staining technique for Fusarium pseudograminearum in cereal tissues during crown rot infections&rft.identifier=http://eprints.usq.edu.au/29371/&rft.publisher=University of Southern Queensland&rft.description=Images of wheat and barley tissues colonised by the pathogens Fusarium pseudograminearum, Blumeria graminis f. sp. hordei and Puccinia striiformis were obtained after staining using a novel technique. This new technique was demonstrated to allow differential staining of host and pathogen tissues in leaves and stems. Several staining combinations were assessed. The selected procedure using solophenyl flavine 7GFE and safranin provided high contrast informative images of fungal and plant structures during pathogenesis.&rft.creator=Noel Knight&rft.date=2016&rft.coverage=159.105459,-9.221084 112.921454,-9.221084 112.921454,-54.777218 159.105459,-54.777218 159.105459,-9.221084&rft_rights=NoLicence&rft_subject=Plant Cell and Molecular Biology&rft_subject=BIOLOGICAL SCIENCES&rft_subject=PLANT BIOLOGY&rft_subject=Mycology&rft_subject=MICROBIOLOGY&rft_subject=Crop and Pasture Protection (Pests, Diseases and Weeds)&rft_subject=AGRICULTURAL AND VETERINARY SCIENCES&rft_subject=CROP AND PASTURE PRODUCTION&rft.type=dataset&rft.language=English Access the data
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Postal Address:
University of Southern Queensland/ Toowoomba, Qld 4350, Australia

noel.knight@usq.edu.au

Brief description

Images of wheat and barley tissues colonised by the pathogens Fusarium pseudograminearum, Blumeria graminis f. sp. hordei and Puccinia striiformis were obtained after staining using a novel technique. This new technique was demonstrated to allow differential staining of host and pathogen tissues in leaves and stems. Several staining combinations were assessed. The selected procedure using solophenyl flavine 7GFE and safranin provided high contrast informative images of fungal and plant structures during pathogenesis.

Notes

1 TIFF image plus document

Available: 21 06 2016

Data time period: 07 2007 to 30 09 2011

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Subjects
Agricultural and Veterinary Sciences | Biological Sciences | Crop and Pasture Production | Crop and Pasture Protection (Pests, Diseases and Weeds) | Microbiology | Mycology | Plant Biology | Plant Cell and Molecular Biology |

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